Peptest is an accurate, painless and inexpensive test that tells you quickly and conclusively if you have reflux disease.
Here’s 10 quick facts on how Peptest works to help patients suffering from reflux conditions.
1. Pepsin should only be in the stomach – if it is found in your saliva, then it means you have refluxed contents from your stomach.
2. We provide customers with tubes to collect saliva samples. The tubes contain citric acid, which acts as a preservative, so you can send samples back to our laboratory for analysis.
3. It takes around 20 minutes to analyse your saliva samples in the Peptest laboratory.
4. We use monoclonal human pepsin antibodies to detect the presence of pepsin in your saliva samples.
5. We will spin your saliva samples in a centrifuge machine for five minutes, at a rate of 4000 rpm, to remove any insoluble material from the saliva and leave a clear supernatant layer (clear fluid layer free of any debris).
6. Then we dilute a sample of this layer with a migration buffer solution, before mixing it in a vortex mixer for around 10 seconds and dropping it into our Peptest device.
7. Our Peptest device is a bit like a pregnancy test and a blue line will appear if there is pepsin present in the saliva.
8. We also measure the concentration or intensity of pepsin in the sample using an electronic lateral flow reader.
9. We will share results with you on the same day as testing your sample – typically this is within 24 hours of a sample arriving at the laboratory.
10. We’ll also send you documentation that you can share with your doctor to tailor the treatment and management of your reflux symptoms.
Hi can you tell me why I need three test tubes for testing the Pepsin in my saliva ,why isn’t one enough ?
Thank You
Hi Jane, This blog post – http://www.peptest.co.uk/how-to-use-peptest-why-we-recommend-a-3-sample-kit/ – explains why we recommend a three sample kit. Hope this helps.
Is it possible to have this test done by you living in another European country?
Thank you.
Hi Dennis,
Many thanks for your question. Yes, you can order Peptest from another European country. Please order directly from the Peptest website: https://www.peptest.co.uk/order/
Thank you.
Hello:
I received my PEPTEST kit in the mail today. Thank you.
I have a question before I begin the sample.
The instructions say to stop certain antacids 48 hours before taking the samples.
I am currently taking 20mg of pantoprazole twice a day morning and evening.
Should I stop taking the medication before beginning the samples?
Thank you for taking the time to answer my question.
V/R
ER
Dear Edward, thank you for your question. You can carry on to take the pantoprazole, it will not effect the test.
Thank you.
I have 2 children I need to test. How many tubes do I need to order?
Hello Charles, thank you for your question.
You will need to order x2 Peptest kits, Peptest kits are provided as either 2 sample (2 tubes) or 3 sample (3 tubes) kits. We recommend the 3 sample kit as this also tests for nocturnal reflux.
Many thanks.
Hi
I’m deperately trying to cure my LPR
I am on Pantoprazole and Gaviscon advance
They are not really doing anything!
Is it ok to use the peptest even thogh I’m on the above pharma drugs?
Thank you
Ceris
Hello Ceris,
Thank you for your your question.
You can proceed to use the Pantoprazole, this will not affect the test however please do not use the Gaviscon Advance for 48 hours before providing the saliva samples and whilst providing the saliva samples.
Many thanks.
So everyone who refluxes will show pepsin enzyme? Does it matter if you have low or high stomach acid? Is that determined by this test ?
Thank you for your comment, Laura.
In answer to the following questions:
“So everyone who refluxes will show pepsin enzyme? Does it matter if you have low or high stomach acid? Is that determined by this test ?”
Pepsin is a digestive enzyme found in the stomach. The presence of pepsin indicates the reflux of gastric contents. This is irrespective of the gastric contents being acidic or non-acidic. Peptest does not determine the acidity of the reflux.
If you have any further questions please do not hesitate to ask.
Can I use your PEPTEST saliva testing system and send the tubes back to you from the United States?
Yes, you can order Peptest – a saliva collection kit and full instructions will be sent direct to your address via FedEx. Once you have collected your samples just call FedEx and they will collect your samples directly from your address and return to our laboratory for analysis. All FedEx courier costs are included in the price. We are working hard to make Peptest available directly within the USA this year.
I’m going on 40mg Nexium (esomeprozole)a day (sometimes 60mg) do I have to stop taking this for 48 hours before doing the test? I am keen to find out what’s happening when I’m on this medication.
Hello Jane,
Thank you for your question.
You can proceed to use the Nexium (esomeprozole), this will not affect the test.
Many thanks.
That should read I’m ON 40mg Nexium…
I had my gallbladder removed 8 months ago. I am now suffering with what I thought was reflux, but no indigestion or heartburn. I was taking omeprazole, with no effect. This was changed to lansoprozole and ranitidine, been taking for 2 weeks and again having no effect. I am now wondering if this is a pepsin issue as I have the feeling of something being in my throat all the time, with soreness and a cough. Does this sound like something this test would help me get to the bottom of?
Dear Sally,
Thank you for your question.
Peptest will detect the presence of Pepsin in your samples. Pepsin may be present in both acidic and non-acidic reflux. Please see our website for further information.
You can order Peptest via our website: http://www.peptest.co.uk by clicking on the order now icon.
Many thanks.
I am in Canada, is that ok?
Hi Jane,
Thank you for your question.
Yes Peptest is available in Canada. You can order Peptest via our website: http://www.peptest.co.uk by clicking on the order now icon.
The cost of Peptest includes all courier delivery costs.
Once we have received your returned samples we will test them in our laboratory in the UK and will then send you a results report via email.
Many thanks.